Question

Which of the following is untrue about DNA sequencing methods?

(a) Purified fragments of DNA cut from plasmid/phage clones or amplified by polymerase chain reaction (PCR)

(b) Clones of DNA fragments are denatured to single strands, and one of the strands is hybridized to an oligonucleotide primer

(c) Taq polymerase is quite heat sensitive

(d) New strands of DNA are synthesized from the end of the primer

I had been asked this question during an interview.

My enquiry is from DNA & Genomic Sequencing topic in chapter Collecting & Storing Sequences in Laboratory of Bioinformatics

Answer 1

The correct answer is (c) Taq polymerase is quite heat sensitive

The best explanation: In an automated procedure, new strands of DNA are synthesized from the end of the primer by heat-resistant Taq polymerase from a pool of deoxyribonucleotide triphosphates (dNTPs) that includes a small amount of one of four chain-terminating nucleotides (ddNTPs).