Question

To sequence larger molecules, individual chromosomes are purified and broken into _____ or larger random fragments, which are cloned into vectors designed for large molecules.

(a) 100-Mb

(b) 100-kb

(c) 5000-kb

(d) 600-kb

I have been asked this question in final exam.

The above asked question is from DNA & Genomic Sequencing in section Collecting & Storing Sequences in Laboratory of Bioinformatics

Answer 1

Right choice is (b) 100-kb

The explanation: To sequence larger molecules, such as human chromosomes, individual chromosomes are purified and broken into 100-kb or larger random fragments, which are cloned into vectors designed for large molecules, such as artificial yeast (YAC) or bacterial (BAC) chromosomes. In a laborious procedure, the resulting library is screened for fragments called contigs, which have overlapping or common sequences, to produce an integrated map of the chromosome.