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Once the correct map has been obtained, unique overlapping clones are chosen for sequencing.

(a) True

(b) False

This question was posed to me during an interview.

My doubt stems from DNA & Genomic Sequencing topic in chapter Collecting & Storing Sequences in Laboratory of Bioinformatics

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Right option is (a) True

The explanation: However, these molecules are too large for direct sequencing. One procedure for sequencing these clones is to subclone them further into smaller fragments that are of sizes suitable for sequencing, make a map of these clones and then sequence overlapping clones. However, this method is expensive because it requires a great deal of time to keep track of all the subclones.

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