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An approach for predicting ______ to look for cases across a set of genomes where _____ are part of the same gene in one genome resulted in gene fusion method.

(a) gene interactions, only three to four orthologs

(b) gene interactions, two orthologs

(c) protein interactions, two or more orthologs

(d) protein interactions, two orthologs

I got this question in exam.

My question comes from The Use of Gene Order & Phylogeny to Predict Protein topic in chapter Global Approaches for Studying Protein – Protein Interactions of Bioinformatics

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Right option is (c) protein interactions, two or more orthologs

Explanation: The prediction is then that the orthologs that are on separate genes in the other genomes interact with each other. In the case of gene fusion, the fused proteins are not only co-regulated, as in conservation of gene order described above, but also permanently colocalized in the cell. The additional requirement of colocalization beyond just co-regulation poses a further limitation on the prediction method.

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