Question

If the bait and prey proteins _______ they bring the DNA-binding and trans-activation domains in such close proximity that they reconstitute the function of the transcription activator, turning ____ the expression of a reporter gene as a result. Which of the following is not the correct pair of blanks?

(a) physically interact, on

(b) do not interact, on

(c) do not interact, off

(d) stop interacting, off

I had been asked this question during an interview.

This interesting question is from Protein Interactions in chapter Functional Genomics & Proteomics of Bioinformatics

Answer 1

Right answer is (b) do not interact, on

The explanation is: Molecular constructs are made such that each of the two domains is covalently attached to each of the two candidate proteins. If the two candidate proteins do not interact, the reporter gene expression remains switched off.