Question

The combined mixture of all labeled DNA fragments is electrophoresed to _____ the fragments by______ and the ladder of fragments is scanned for the presence of each of the four labels.

(a) separate, size

(b) separate, pH

(c) assimilate, pH

(d) assimilate, size

I got this question in an international level competition.

This intriguing question originated from DNA & Genomic Sequencing topic in section Collecting & Storing Sequences in Laboratory of Bioinformatics

Answer 1

The correct choice is (a) separate, size

To explain I would say: A computer program then determines the probable order of the bands and predicts the sequence. Depending on the actual procedure being used, one run may generate a reliable sequence of as many as 500 nucleotides.